Note: This information will be effective in August 1996
CREDIT BREAKDOWN:
Credit: 3
Lecture: 3
Lab: 0
Practicum: 0
Work experience: 0
COURSE DESCRIPTION:
Introductory genetics course for Biology and Biotechnology majors. Topics
covered include: DNA and chromosome structure and function; Mendelian genetics;
Molecular genetics in eucaryotes, bacteria and viruses; Recombinant DNA
Technology; Gene expression;and the Genetic Basis of Immunology.
PREREQUISITES: BIOL 141; HS Chemistry and credit or enrollment in
CHEM 131 or CHEM 151
COURSE COMPETENCIES:
During this course, the student will be expected to:
1. Describe the science of heredity and genetic manipulation.
1.1 Discuss the historical aspects of genetics, from Hippocrates and Aristotle to Darwin.2. Summarize the process of cell division, and chromosome structure and function.
1.2 Explain basic concepts of genetics.
1.3 Discuss investigative methods used in genetics and explain the importance and extent of their application.
2.1 Describe the process of meiosis and mitosis.3. Discuss the history and significance of Mendel's work in genetics.
2.2 Distinguish between meiosis and mitosis.
2.3 Review the importance of the processes of meiosis and mitosis.
3.1 Explain monohybrid and dihybrid crosses.4. Summarize factors known to modify simple Mendelian Ratios.
3.2 Analyze monohybrid and dihybrid crosses using test crosses.
3.3 Demonstrate the use of Punnett Squares in the analysis of monohybrid and dihybrid crosses and test crosses.
3.4 Summarize Mendel's principles of segregation and independent assortment.
3.5 Probability and Genetic Events
3.6 Interpret genetic data using Chi-square analysis.
3.7 Human Pedigrees
4.1 Identify and use appropriate symbols for alleles.5. Discuss linkage vs. independent assortment and chromosome mapping.
4.2 Discuss multiple alleles, incomplete dominance, codominance, and sex-linked inheritance.
5.1 Distinguish between incomplete and complete linkage.6. Summarize the characteristics and function of genetic material.
5.2 Explain the process and importance of crossing over.
5.3 Review principles of segregation and independent assortment.
5.4 Explain the three criteria for a successful mapping cross.
5.5 Identify the gene sequence in a mapping cross.
5.6 Diagram a mapping problem.
5.7 Identify the gene sequence from a genetic map.
6.1 Discuss the history of evidence that indicated DNA was responsible for transformation of bacteria and that DNA was also the genetic material in eucaryotes.7. Describe the process of DNA replication and synthesis.
6.2 Explain the role of bacteriophages in transfection.
6.3 Review DNA structure, including the Watson-Crick model.
6.4 Review the three classes of RNA and their functions.
6.5 Examine the principles of molecular hybridization and reassociation kinetics.
7.1 Explain the roles of DNA polymerase I, II and III.8. Summarize the processes of DNA transcription and translation.
7.2 Discuss continuous and discontinuous DNA synthesis.
7.3 Compare procaryotic vs. eucaryotic DNA synthesis.
8.1 Review the genetic code.9. Discuss protein structure and function.
8.2 Describe the initial studies that enabled scientists to decipher the genetic code.
8.3 Explain codons and anticodons.
8.4 Discuss degeneracy and wobble in the genetic code.
8.5 Summarize universality of the genetic code.
8.6 Compare the processes of transcription and translation in procaryotes vs. eucaryotes.
9.1 Review the one gene - one enzyme and one gene - one protein hypotheses and discuss their historical importance.10. Discuss genetic variation, its' origin and importance.
9.2 Explain protein structure.
9.3 Describe protein function.
10.1 Classify mutations .11. Compare DNA organization in viruses, bacteria, mitochondria, chloroplasts and eucaryotic nuclei.
10.2 Discuss methods for detecting mutations in bacteria, fungi, Drosophila, and in humans.
10.3 Describe the molecular basis for genetic mutations.
10.4 Explain the use of the Ames Test in detection of mutagens.
10.5 Review the role of radiation in DNA damage and repair.
10.6 Discuss the role of transposons in genetic mutation/variation.
11.1 Review the organization and function of mitochondrial DNA.12. Explain the genetics of bacteria and bacteriophages.
11.2 Review the organization and function of chloroplast DNA.
11.3 Describe the organization of DNA in eucaryotic chromatin.
11.4 Relate the importance of chromosome banding techniques.
11.5 Review eucaryotic genome structure.
11.6 Review structure of viral and bacterial chromosomes.
12.1 Review general principles of bacterial growth and mutation.13. Summarize recombinant DNA technology.
12.2 Discuss conjugation in bacteria.
12.3 Discuss the role of plasmids in bacterial mutations and Recombinant DNA research.
12.4 Explain transposons, insertion sequences and inverted repeats.
12.5 Describe bacterial transformation.
12.6 Discuss the role of bacteriophages in transformation.
12.7 Explain the nature and importance of transduction.
12.8 Discuss reverse transcriptase and retroviruses, including their role in oncogenesis.
12.9 Review AIDS and discuss HIV as a subgroup of retroviruses.
13.1 Explain the role of restriction enzymes.14. Summarize the regulatory mechanisms of gene expression in procaryotes and eucaryotes.
13.2 Discuss the types of vectors and how they are used.
13.3 Relate the construction and importance of genomic, chromosome-specific and cDNA libraries.
13.4 Explain the selection of recombinant clones.
13.5 Describe PCR Analysis and explain its' importance.
13.6 Explain the characterization of cloned DNA sequences using restriction mapping, nucleic acid blotting and DNA sequencing.
13.7 Discuss the importance of various applications of recombinant DNA technology, including gene mapping, diagnostics, DNA fingerprinting, gene therapy and protein production.
13.8 Discuss the scientific, economic, social and ethical aspects of the Human Genome Project and of recombinant DNA technology in general.
14.1 Distinguish between models for positive and negative control of gene expression.15. Explain the function and regulation of the various components of the immune system.
14.2 Explain the regulation of lactose metabolism in E. coli.
14.3 Explain repressible genes and attenuation.
14.4 Distinguish between the lysogenic and lytic pathways used by bacteriophage Lambda in E. coli.
14.5 Review regulation of transcription in eucaryotes.
14.6 Discuss posttranscriptional regulation in eucaryotes.
15.1 Discuss antibody-mediated and cell-mediated immunity, and the concept of immunological memory.16. Population Genetics
15.2 Review theories of antibody formation.
15.3 Relate organization and recombination of immunoglobulin genes to antibody diversity.
15.4 Demonstrate understanding of production of monoclonal antibodies and their applications.
15.5 Discuss the ABO blood group system and Rh compatibility.
15.6 Explain histocompatibility antigens.
15.7 Discuss the role of HLA antigens and MHC genes in governing the success of tissue and organ transplantation.
15.8 Discuss disorders of the immune system, including AIDS and autoimmune diseases.
16.1 Populations, Gene Pools, and Allele Frequencies
16.2 The Hardy-Weinberg Law
16.3 Factors that Alter Allele Frequencies