Chapter 5 Links
Methods of DNA Technology

Vectors

• Cloning and Cloning Vectors
  • This is a good site about vectors. The kinds of vectors discussed are plamids, bacteriophage lambda vectors, cosmids, and yeast artificial chromosomes (YACs). The site finishes off with information on library screening and gene sequencing.

Plasmid

• Plasmids
  • This site focuses on using plasmids as vectors.
• Plasmid Insertion
  • View a colorful diagram demonstrating plasmid insertion.

Chimeras

• Origin, Development, and Propogation of Chimeras
  • The focus here is on chimeral plants. Unfortunately this site is a bit technical.

Virus

• All the Virology on the WWW
  • All the Virology on the WWW seeks to be one of the best single sites for Virology information on the internet. (Highly recommended!)
• Institute for Molecular Virology
  • Also highly recommended. Check out the computer visualizations of viruses in their Multimedia Library.
• THE BIG PICTURE BOOK OF VIRUSES
  • By D.M. Sander, Garry Laboratory, Dept. of Microbiology and Immunology, School of Medicine at Tulane U. Nice photos.
• Nucleic Acid: List of ICTV approved Virus Families and Genera
  • From the Australian National University.
• Virus Genomes: Structure & Complexity of Virus Genomes
  • Nice overview from a microbiology course at the U of Leicester in the U.K.
• Dr. Frederick A. Murphy Talks about the Ebola Virus
  • An interview and links from Access Excellence.
• Outbreak
  • An on-line information service addressing emerging diseases. The latest breaking news on Ebola and other viruses. Check it out.
• Virology Information
  • This site has complete information on viruses. It has an interesting section on new developments in the fight against viruses.
• Viruses and Cancer
  • Describes some specific viruses relating to cancer.
• Xiong's Virology Page
  • This site focuses on plant viruses. Of interest to us is Xiong's information on taxonomy and description of plant viruses, and information on his research projects.

Retrovirus

• Retroviruses
  • This excellent site discusses everything you want to know about retroviruses - that includes taxonomy, structure, genome, reverse transcription, gene expression, and pathogenesis. The great plus of this site are numerous colorful diagrams and an activity where you can pretend to be a retrovirus.
• Retroviruses: An Introduction
  • A historical perspective of retroviruses.
• Retrovirus Replication
  • Here is a neat diagram from Access Excellence.
• Human Retrovirus Section
  • The Human Retrovirus Section of the National Cancer Institute performs basic research on the molecular biology of gene expression, on the biology of human retroviruses and the pathogenesis of AIDS. Very research oriented material.

Viral-Vector Vaccines

• Vaccines
  • Don't expect this site to discuss only viral-vector vaccines, but expect a complete discussion on all vaccines.
• Biotechnology and Vaccine Development
  • This site has even more information on vaccines including historical development, recombinant vaccines, live vectors, vaccines based on nucleic acids, peptides as vaccines, vaccines and the immune response, and cytokines.
• Vaccines
  • The focus here is on viral vaccines.

Bacteriophage

• Bacteriophage T4 VRML Visualizations
  • This has a good introduction to T4 and it has neat visualizations.
• Bacteriophage T4 Home Page
  • Check out their current research section.
• Bacteriophage Ecology Group
  • Dedicated to the elucidation of the ecology and evolutionary biology of the parasites of unicellular organisms.
• Bacteriophage Lambda Vectors
  • This site discusses how phages are used as vectors.
• Computer Visualizations of Viruses - Examples
  • See computer visualizations of two bacteriophages.

Escherichia coli

• Bugs in the News - What the Heck is an E. coli?
  • E. coli info from the great biology education resources of the U of Kansas.
• The E. coli Index
  • A comprehensive guide to information relating to the bacterium Escherichia coli on the WWW.
• Escherichia coli
  • Here there are two pictures of E. coli growing on eosin methylene blue (EMB) agar.
• E. coli 0157:H7 Infection
  • Similar to the "What the Heck is.." site, but it provides extra information.
• Preventing Foodborne Illness: Escherichia coli O157:H7
  • Questions and answers from the CDC.
• E. Coli Primer
  • E. coli infection from a legal perspective.

Saccharomyces cerevisiae

• What are yeasts?
  • An introduction to all types of yeast.
• Molecular Biology of Brewers Yeast
  • A good site with many good links.
• Yeast Molecular Genetics
  • A brief description into the use of yeast in experiments.
• You Can & Yeast
  • Highly recommended! This site explains how yeast makes bread rise and it tells you how to grow yeast and make bread.
• Yeast Experiments
  • These articles describe genetics and radiation experiments using yeast (Saccharomyces cerevisiae), a popular eukaryotic microbe for genetics and molecular biology research. They include student experiments and techniques, teacher tips, background material, integrated applications to environmental science, math, and physics, and sources of supplies.

Replica Plating

• Replica Plating
  • This site gives a brief description of the technique.
• Replica Plating Equipment
  • Several photos of the replica plating equipment.