Use the following information and the figure below to answer the questions. The plasmid pGEN308, a 18 kb circular piece of DNA, was treated with various mixtures of restriction enzymes. The two endonucleases were HindIII and Bgl I (pronounced "Hin-dee three" and "Bagel one" respectively). pGEN308 has four of the relevant sites: 3 Bgl I's (at approx. 3, 5 and 8 o'clock) and one HindIII at 12 o'clock). The other plasmid information is extraneous but was left in to give you an idea of some of the complexity found in plasmids. Size or distances (in kb of DNA) are given between each restriction site. They are not drawn to actual scale.
Your job is to draw a picture of the DNA fingerprinting gel obtained after digesting the plasmid with various enzyme "cocktails" and separating the DNA fragments using agarose gel electrophoresis. The migration distance of any DNA fragment is proportional to the size of the fragment. Your gel should be oriented vertically with "-" electrode at the top and "+" at the bottom. It should contain 4 lanes. They are: I, the standard size marker lane with Lambda bacteriophage fragments of 20, 11, 8, 6 and 3 kb size; lane II, fragments that might result from using HindIII alone; lane III, fragments that might result from using Bgl I alone; and lane IV, fragments that might result from using both Bgl I and Hind III together. Lanes may be designated from left to right as L, H, B, and B/H. In each lane, indicate the size of each fragment in kb next to the appropriate band.
Total value of this part of the DNA Fingerprinting assignment: 20 pts.
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